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2019-nCoV Molecular Diagnostic Kit (Fluorescent PCR Test) (Lyophilized)

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2019-nCoV Molecular Diagnostic Kit (Fluorescent PCR Test) (Lyophilized)

Model Number : GE88-01

Certification : CE

MOQ : 1000 test

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2019-nCoV Molecular Diagnostic Kit (Fluorescent PCR Test) (Lyophilized)

The 2019-nCoV Molecular Diagnostic Kit is a TaqMan probe real-time fluorescent PCR to qualitatively detect ORF1ab and N genes nucleic acid of 2019-nCoV in throat swab, saliva in patients with respiratory infection symptoms and close contacts.

2019-nCoV Molecular Diagnostic Kit (Fluorescent PCR Test) (Lyophilized)

The test results are for clinical reference only and cannot be used as a basis for confirming or eliminating cases. Operators should have experience of professional training in gene amplification or molecular biology method testing, and have relevant qualifications for laboratory operations. The laboratory should have reasonable biological safety precautions and protective procedures.

In this kit, primers and probes of real-time fluorescent PCR technology are designed to the conserved and specific regions of the ORF1ab and N genes of 2019-nCoV, respectively. During PCR amplification, the probe binds to the template, and the 5'-end reporter group of the probe is cleaved by the Taq enzyme (5'3' exonuclease activity), thereby moving away from the quenching group to generate a fluorescent signal. The real-time amplification curve is automatically plotted based on the detected fluorescence signal, and the sample Ct value is calculated. FAM and HEX fluorophores are labeled to ORF1ab gene and N gene probes in 2019-nCoV-PCR reaction solution. By using one test, qualitative detection of the above two genes of 2019-nCoV can be performed simultaneously.

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